Cultivation of embryos is a direct support for the healthy development of the embryos, which were obtained as a result of the in vitro fertilization program (during the preimplantation period). We use remarkably unique medias for the cultivation process. They are containing a complex of nutrients that contributes to the full development of the embryo, it stimulates the protective function of the cells and increases the proliferation potential.
Prolonged cultivation of embryos — 5-6 days — is the "golden standard" of the international practice in embryology. During this period, the embryo reaches the blastocyst stage. This stage is considered to be perfect for evaluation of the embryo's viability and perspectiveness of the following transfer into the body. Prolonged cultivation of embryos also provides additional opportunities for preimplantational genetic diagnosis (PGD).
ICSI — intracytoplasmic sperm injection into the oocyte — is a special technique of assisted reproductive technology, in which the embryologist carries out the fertilization for an oocyte by specially chosen "the best" sperm cell. "The best" is pre-selected by means of high-tech optical equipment taking into account its appearence and other features. ICSI allows to make fertilization even when only single barely motile spermatozoa are present.
SMART — Sperm Motility Activating Rescue Technology — is a technique allowing to make motile completely or barely immobile sperm, which has been obtained from testicles during surgical operation. Such technique is also so-called "sperm motility advanced treatment".
It can happen that in a result of fertilization, there are formed multiple life-promising and simply perspective embryos. If those embryos are not planned to be transferred during the ongoing IVF program treatment, it is recommended to make a cryopreservation of potent embryos, or simply freeze them. In the future, they can be used to achieve the repeated pregnancies, as well as to simplify the next treatment protocol.
Vitrification is a modern, specific secure method of freezing cells. It allows to preserve oocytes, which were obtained as a result of puncture. Vitrificated oocytes could be used for multiple IVF programs without re-stimulation of ovulation and operative extraction of the follicles. Vitrification of oocytes up to 30 years is considered as the "insurance" of infertility since it makes possible postponed successful motherhood in the late reproductive age.
Cryopreservation is a medical tag for the semen freezing. It is a special technique of male gametes cryoconservation with following cryopreservation. It makes possible such things as delayed parenthood, fertility preservation before cancer treatment therapy, simplified process of repeated IVF programs (if the first treatment cycle failed), as well as to achieve the repeated pregnancies.
Vitrification of ovarian tissues is a great breakthrough in modern medicine. The technique makes it possible to preserve fertility for patients with oncologies when there is no time to procced with ovulation stimulation and follicle puncture. Tissue autotransplantation after cancer treatment therapy back into woman's body results in restoration of the whole hormonal system with normal menstruation and ovulation.
Assisted fermentative hatching is an extremely precise micromanipulation carried out by embriologist under a microscope. The aim is to create a small hole in the "shiny layer" of the embryo (zona pellucida). It is made on the fourth day of embryo development, when the embryo contains an average of six to eight cells. This manipulation is made prior to embryo transfer into uterine cavity of a woman.
Oocyte in vitro maturation (IVM) is an assisted reproductive technique, in which oocytes are retrieved from the follicles of unstimulated or minimally stimulated ovaries. IVM of human oocytes is an efficient procedure since it has advantages over controlled ovarian stimulation such as reduction of a price, simplicity, and elimination of ovarian hyperstimulation syndrome (by reduction of gonadotropin stimulation). IVM offers eligible infertile couples a safe and convenient form of treatment.
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